Fluorogenic protein tags for live-cell imaging
Stain different, tag FAST with The Twinkle Factory Request a quoteFor TTOsThe Twinkle Factory is rooted in the wish of Arnaud Gautier, Ludovic Jullien and Franck Perez, three top-notch scientists of ENS-PSL, Sorbonne University, and Institut Curie to help life science researchers accelerate their work. The Twinkle Factory thus distributes reagents for their breakthrough protein fluorescent reporters and molecular glues, FAST and splitFAST (What is it, FAST and splitFAST?), and CATCHFIRE (What is it, CATCHFIRE?).
It all started in 2019 with a three-item-long pricelist for FAST and splitFAST. Along with cofounders’ research, non-permeant fluorogens, a far-red fluorogen, a fluorescence quencher, have added to the list. As a result, sales rapidly expanded worldwide. So far, 300 research teams at universities, life sciences startups, big pharmas, CROs, have implemented FAST and splitFAST in their research. Their key words are metabolic engineering, microbiota, protein-protein interactions, synthetic biology, cell-based assays, sensors… Furthermore, they unveiled the potential of FAST in hypoxic conditions, equipping a vast array of microorganisms.
And in 2023, we have added a whole range of fluorogenic molecular glues for CATCHFIRE.
This is just the beginning! Actinometers, and many other twinkling reagents are indeed joining the pack. Stay tuned!
Our latest post
RSpFAST: a FAST tag you can switch with light
A new fluorogen turns the pFAST tag into a reversible photoswitch, and that opens up imaging you cannot do with an ordinary green label. RSpFAST is not a new tag. It is the pFAST tag paired with a fluorogen that light can flip between two forms. Bound inside pFAST,...
Our earlier posts
FAST and splitFAST, greenFAST & redFAST, pFAST in a nutshell
A review of this exciting journey in Account Chemistry Research by inventor Prof. Arnaud Gautier
FAST and splitFAST to the fore at CLOSTRIDIUM XVI
three talks and one poster reviewing FAST of The Twinkle Factory in acetogenic and solventogenic Clostridia
FAST in archaea imaging: towards a new standard
FAST in anaerobic methanogenic archaea FACSing just reported in bioRxiv by N. Adlung of Uni Aalto
Microbiota in vivo imaging with FAST-encoded bacterial probes
Gut and tumor microbiota in vivo imaging disclosed by a team of the Shanghai Jiao Tong University
FAST and splitFAST in Archaea for the first time ever!
FAST and splitFAST in Archaea just disclosed by Kyle Costa’s lab at University of Minnesota
The Twinkle Factory launches the non-permeant fluorogen tfFox-NP
For protein trafficking / excretion studies – After A. Gautier in Nat. Commun. 2021
The Twinkle Factory launches the quencher tfDarth
For your most demanding multi-step multi-color protocols – after Arnaud Gautier in Nat. Commun. 2021
Speed up lactate-producing strain engineering with FAST
A collaboration of Ulm University and the University of Nottingham published in Applied Microbiology and Biotechnology
greenFAST and redFAST in anaerobes
Towards #CarbonSmart – by M. Flaiz of Ulm University – in ACS Synth. Biol.
Painting with pFAST, a FAST variant, for advanced imaging
In Nat. Commun., Prof. Arnaud Gautier discloses an improved pFAST and a variety of novel fluorogens
US patent for non-permeant FAST fluorogens just granted
The USPTO has just granted the patent for non-permeant FAST fluorogens by Prof. Arnaud Gautier et al.
First-ever report of FAST in anaerobic Giardia intestinalis
In BMC Biology by Charles University and Ludwig-Maximilians University researchers
Thanks to the continued support of PSL Université and Région Ile-de-France.