Fluorogenic protein tags for live-cell imaging
Stain different, tag FAST with The Twinkle Factory Request a quoteThe Twinkle Factory is rooted in the wish of Arnaud Gautier, Ludovic Jullien and Franck Perez, three top-notch scientists of ENS-PSL, Sorbonne University, and Institut Curie to help life science researchers accelerate their work. The Twinkle Factory thus distributes reagents for their breakthrough protein fluorescent reporters and molecular glues, FAST and splitFAST (What is it, FAST and splitFAST?), and CATCHFIRE (What is it, CATCHFIRE?).
It all started in 2019 with a three-item-long pricelist for FAST and splitFAST. Along with cofounders’ research, non-permeant fluorogens, a far-red fluorogen, a fluorescence quencher, have added to the list. As a result, sales rapidly expanded worldwide. So far, 300 research teams at universities, life sciences startups, big pharmas, CROs, have implemented FAST and splitFAST in their research. Their key words are metabolic engineering, microbiota, protein-protein interactions, synthetic biology, cell-based assays, sensors… Furthermore, they unveiled the potential of FAST in hypoxic conditions, equipping a vast array of microorganisms.
And in 2023, we have added a whole range of fluorogenic molecular glues for CATCHFIRE.
This is just the beginning! Actinometers, and many other twinkling reagents are indeed joining the pack. Stay tuned!
Our latest post
CATCHFIRE: a reversible switch for enzyme activity and gene expression
Turn a protein’s activity on, then off again, with a single small molecule. A new study turns CATCHFIRE into a versatile chemical switch for controlling enzymes and gene expression, reversibly and on demand. The work comes from the teams of Prof. Arnaud Gautier...
Our earlier posts
FAST and splitFAST in Archaea for the first time ever!
FAST and splitFAST in Archaea just disclosed by Kyle Costa’s lab at University of Minnesota
The Twinkle Factory launches the non-permeant fluorogen tfFox-NP
For protein trafficking / excretion studies – After A. Gautier in Nat. Commun. 2021
The Twinkle Factory launches the quencher tfDarth
For your most demanding multi-step multi-color protocols – after Arnaud Gautier in Nat. Commun. 2021
Speed up lactate-producing strain engineering with FAST
A collaboration of Ulm University and the University of Nottingham published in Applied Microbiology and Biotechnology
greenFAST and redFAST in anaerobes
Towards #CarbonSmart – by M. Flaiz of Ulm University – in ACS Synth. Biol.
Painting with pFAST, a FAST variant, for advanced imaging
In Nat. Commun., Prof. Arnaud Gautier discloses an improved pFAST and a variety of novel fluorogens
US patent for non-permeant FAST fluorogens just granted
The USPTO has just granted the patent for non-permeant FAST fluorogens by Prof. Arnaud Gautier et al.
First-ever report of FAST in anaerobic Giardia intestinalis
In BMC Biology by Charles University and Ludwig-Maximilians University researchers
FAST to explore multi-species low-oxygen biofilms
In this npj Biofilms Microbiomes issue, Prof. Henry builds up FAST experience for biofilms studies
Monitoring butanol production with FAST in acetogenic bacteria
FAST in Eubacterium limosum by Prof. Dr. Peter Dürre of Ulm University – Biotechnology for Biofuels
Metabolic engineering in Saccharomyces cerevisiae with FAST
Adding Saccharomyces cerevisiae to the array of FAST and splitFAST applications
What about FAST for anaerobic protozoa fluorescence labeling?
Struggling for florescence labeling of parasitic protozoa? Some food for thought at the onset of a new year
Thanks to the continued support of PSL Université and Région Ile-de-France.