Stain different, tag FAST
Stain Different Tag FAST with The Twinkle Factory! This is how everything got started in 2017 from the wish of Arnaud Gautier, Ludovic Jullien and Franck Perez, three top-notch scientists from ENS-PSL, Sorbonne University, and the Institut Curie to help others accelerate their research.
Seeing cellular mechanisms is the first step towards understanding the inner workings of living systems. But many biological molecules and processes are yet to be unveiled. Our research aims at inventing new ways of observing biomolecules and cellular events via next-generation fluorescent labels in cell-based assays, live cell imaging, cytometry.
The Twinkle Factory’s products are based on the instantaneous formation of a fluorescent molecular assembly between the FAST protein or splitFAST and fluorogenic ligands (Fluorogens). Only the assembly of both the tag and the Fluorogen fluoresce, allowing specific detection by fluorimetry or optical microscopy. We hence provide efficient tools for various applications in biological imaging, from fundamental mechanisms to drug screening.
Our latest post
FAST and splitFAST in Archaea for the first time ever!
FAST and splitFAST in Archaea! A preprint in bioRxiv 2022 just reports a team of the University of Minnesota implementing those in Methanococcus maripaludis. Furthering bacteria and eukaryots, this step opens a brand new domain for The Twinkle Factory. Prof. Terry...
Our earlier posts
The Twinkle Factory launches the non-permeant fluorogen tfFox-NP
For protein trafficking / excretion studies – After A. Gautier in Nat. Commun. 2021
The Twinkle Factory launches the dark chromophore tfDarth
For your most demanding multi-step multi-color protocols – after Arnaud Gautier in Nat. Commun. 2021
Speed up lactate-producing strain engineering with FAST
A collaboration of Ulm University and the University of Nottingham published in Applied Microbiology and Biotechnology
greenFAST and redFAST in anaerobes
Towards #CarbonSmart – by M. Flaiz of Ulm University – in ACS Synth. Biol.
Painting with pFAST, a FAST variant, for advanced imaging
In Nat. Commun., Prof. Arnaud Gautier discloses an improved pFAST and a variety of novel fluorogens
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US patent for non-permeant FAST fluorogens just granted
The USPTO has just granted the patent for non-permeant FAST fluorogens by Prof. Arnaud Gautier et al.
First-ever report of FAST in anaerobic Giardia intestinalis
In BMC Biology by Charles University and Ludwig-Maximilians University researchers
FAST to explore multi-species low-oxygen biofilms
In this npj Biofilms Microbiomes issue, Prof. Henry builds up FAST experience for biofilms studies
Monitoring butanol production with FAST in acetogenic bacteria
FAST in Eubacterium limosum by Prof. Dr. Peter Dürre of Ulm University – Biotechnology for Biofuels
Metabolic engineering in Saccharomyces cerevisiae with FAST
Adding Saccharomyces cerevisiae to the array of FAST and splitFAST applications
What about FAST for anaerobic protozoa fluorescence labeling?
Struggling for florescence labeling of parasitic protozoa? Some food for thought at the onset of a new year