The Twinkle Factory Fluorogens

Our fluorogens

We are offering numerous Fluorogens for FAST and splitFAST, in a large variety of colors and other properties. Here is our catalogue.

The Twinkle’s catalogue

The Fluorogens for FAST and splitFAST

The Twinkle Factory offers a variety of commercial Fluorogens for FAST and splitFAST differing by their color (emission wavelength), their brightness (quantum yield), and their affinity for the tag. They also may be non-permeant through mammalian cell membranes (NP).

Most of our commercial Fluorogens can be used with any version of FAST (i.e., FAST1, FAST2, pFAST, tdFAST1, tdFAST2, frFAST) or splitFAST. greenFAST is specific for ^TFLime and redFAST for ^TFCoral. Used together, they hence allow multicolor orthogonal labeling. frFAST was specifically designed with ^TFPoppy for far-red labeling.

More than 40 plasmids for FAST and splitFAST in mammalian cells have been made available on Addgene by Prof. Arnaud Gautier so to help expedite your research.

Beyond, we have been partnering with e-Zyvec, the “DNA-vectors-made-easy” company. Based on their proprietary design technology, they offer an integrated service for design and production of tailor-made DNA vectors. They have included FAST and splitFAST into D-Zyvec, their powerful design engine.

FAST tags X Fluorogens

	FAST1	FAST2	pFAST	tdFAST1	tdFAST2	splitFAST	redFAST	greenFAST	frFAST	split-frFAST
	a.k.a. Y-FAST	a.k.a. iFAST	optimized FAST	FAST1 tandem	FAST2 tandem	FAST1 split	for red labelling	for green labelling	for far-red labelling	split for far-red labelling
^TFLime a.k.a. HMBR 480-541 nm	+++	+++	+++	+++	+++	+++	0	+++	+	+	PNAS 2016
^TFAmber a.k.a. HBR-3,5DM 499-558 nm	+++	+++	+++	+++	+++	+++	0	0	+	+	Chem. Sci. 2017
^TFAmber-NP a.k.a. HBRAA-3E 505-559 nm non permeant	+++	+++	+++	+++	+++	+++	0	0	+	+	Bioconjug. Chem. 2018
^TFFox-NP a.k.a. HBRAA-3,5DM 524-578 nm non permeant	n.d.	n.d.	+++	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.	Nat. Commun. 2021
^TFCoral a.k.a. HBR-3,5DOM 516-600 nm	+++	+++	+++	+++	+++	+++	+++	0	+	+	Chem. Sci. 2017
^TFPoppy a.k.a. HPAR-3OM 555-670 nm	0	0	0	0	0	0	0	0	+++	+++	Angew. Chem. Int. Ed. 2020
^TFDarth a.k.a. HBIR-3M non fluorescent	n.d.	n.d.	+++	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.	Nat. Commun. 2021
	PNAS 2016	Biochemistry 2018	Nat. Commun. 2021	Biochemistry 2018	Biochemistry 2018	Nat. Commun. 2021	Nat. Chem. Biol. 2020	Nat. Chem. Biol. 2020	Angew. Chem. Int. Ed. 2020
		+++ perfect fit		+ average		0 NOK		n.d. no data

This table allows for selecting the best couple FAST tag X Fluorogen depending on your application

Open as PDF

^TFLime – 480541-250

LIME

a.k.a. HMBR

Absorption and emission wavelengths when bound to
FAST1: 481 / 540 nm – FAST2: 480 / 541 nm – frFAST : 484 / 550 nm

Molar absorption coefficient when bound to
FAST1: 45,000 – FAST2: 41,000 – frFAST: 56,000 M–1cm–1

Fluorescence quantum yield when bound to
FAST1: 23 % – FAST2: 23 % – frFAST: 13%

Affinity constant at 25°C of its complex with
FAST1: 0.13 µM – FAST2: 0.07 µM – frFAST: 0.50 µM

Download Lime Datasheet

To be used with:

FAST1, a.k.a. Y-FAST – more about FAST1 sequence

FAST2, a.k.a. iFAST – more about FAST2 sequence

tdFAST1 – more about tdFAST1 sequence

tdFAST2 – more about tdFAST2 sequence

frFAST – more about frFAST sequence

greenFAST – more about greenFAST sequence

^TFAmber – 499558-250

AMBER

a.k.a. HBR-3,5DM

Absorption and emission wavelengths when bound to
FAST1: 499 / 562 nm – FAST2: 499 / 558 nm

Molar absorption coefficient when bound to
FAST1: 48,000 – FAST2: 46,000 – frFAST: 55,000 M–1cm–1

Fluorescence quantum yield when bound to
FAST1: 49 % – FAST2: 57 % – frFAST: 50%

Affinity constant at 25°C of its complex with
FAST1: 0.08 µM – FAST2: 0.06 µM – frFAST: 0.80 µM

Download Amber Datasheet

To be used with:

FAST1, a.k.a. Y-FAST – more about FAST1 sequence

FAST2, a.k.a. iFAST – more about FAST2 sequence

tdFAST1 – more about tdFAST1 sequence

tdFAST2 – more about tdFAST2 sequence

^TFAmber-NP – 505559-250

AMBER-NP

a.k.a. HBRAA-3E

Absorption and emission wavelengths when bound to
FAST1: 505 / 559 nm

Molar absorption coefficient when bound to
FAST1: 61,000 M–1cm–1

Fluorescence quantum yield when bound to
FAST1: 8 %

Affinity constant at 25°C of its complex with
FAST1: 1.3 µM

Download Amber-NP Datasheet

To be used with:

FAST1, a.k.a. Y-FAST – more about FAST1 sequence

tdFAST1 – more about tdFAST1 sequence

^TFCoral – 516600-250

CORAL

a.k.a. HBR-3,5DOM

Absorption and emission wavelengths when bound to
FAST1: 518 / 600 nm – FAST2: 516 / 600 nm – frFAST: 525 / 600 nm

Molar absorption coefficient when bound to
FAST1: 39,000 – FAST2: 38,000 – frFAST: 51,000 M–1cm–1

Fluorescence quantum yield when bound to
FAST1: 31 % – FAST2: 40 % – frFAST: 19%

Affinity constant at 25°C of its complex with
FAST1: 0.97 µM – FAST2: 0.41 µM – frFAST: 3.9 µM

Download Coral Datasheet

To be used with:

FAST1, a.k.a. Y-FAST – more about FAST1 sequence

FAST2, a.k.a. iFAST – more about FAST2 sequence

tdFAST1 – more about tdFAST1 sequence

tdFAST2 – more about tdFAST2 sequence

frFAST – more about frFAST sequence

redFAST – more about redFAST sequence

^TFPOPPY – 555670-250

POPPY

a.k.a. HPAR-3OM

Absorption and emission wavelengths when bound to
frFAST: 555 / 670 nm

Molar absorption coefficient when bound to
frFAST: 45,000 M–1cm–1

Fluorescence quantum yield when bound to
frFAST: 21 %

Affinity constant at 25°C of its complex with
frFAST: 1.0 µM

Download Poppy Datasheet

To be used with:

frFAST – more about frFAST sequence

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