The Twinkle Factory reagents

fluorogens AND Molecular glues

We are offering a number of Fluorogens for FAST and splitFAST, and molecular glues for CATCHFIRE in a large variety of colors and other properties

The Twinkle Factory offers two ranges of reagents differing by their color (emission wavelength), their brightness (quantum yield), and their affinity for their cognate tags:

  • Fluorogens for FAST and splitFAST
  • Molecular glues for CATCHFIRE

More than 60 plasmids for FAST and splitFAST, and CATCHFIRE, in mammalian cells have been made available on Addgene by Prof. Arnaud Gautier so to help expedite your research.  Beyond, our partner e-Zyvec (Polyplus, part of Sartorius) offer an integrated service for design and production of tailor-made DNA vectors.  They have included FAST and splitFAST into D-Zyvec, their powerful design engine.

 The Twinkle’s catalogue

Molecular glues for CATCHFIRE

match540

ref: match540-50X

a fluorogenic dimerizer emitting at 540 nm

match550

ref: match550-50X

the most powerful of our fluorogenic dimerizers, emitting at 550 nm

match600

ref: match600-50X

a fluorogenic dimerizer emitting at 600 nm

 matchDark

ref: matchDark-50X

a non-fluorogenic (dark), the most powerful dimerizer known for CATCHFIRE

cognate proteic tags

 All above molecular glues have been optimized for FIREmate and FIREtag

 The Twinkle’s catalogue

Fluorogens for FAST and splitFAST

The Twinkle Factory offers a variety of commercial Fluorogens for FAST and splitFAST differing by their color (emission wavelength), their brightness (quantum yield), and their affinity for the tag. They also may be non-permeant through cell membranes (the NP series).

TFLime

ref: 480541-250

A greenish, membrane-permeant, fluorogenic ligand

a.k.a. HMBR

Absorption and emission wavelengths when bound to
FAST1: 481 / 540 nm – FAST2: 480 / 541 nm – frFAST : 484 / 550 nm

Molar absorption coefficient when bound to
FAST1: 45,000 – FAST2: 41,000 – frFAST: 56,000 M–1cm–1

Fluorescence quantum yield when bound to
FAST1: 23 % – FAST2: 23 % – frFAST: 13%

Affinity constant at 25°C of its complex with
FAST1: 0.13 µM – FAST2: 0.07 µM – frFAST: 0.50 µM

TFAmber

ref: 499558-250

A yellowish, membrane-permeant, fluorogenic ligand

a.k.a. HBR-3,5DM

Absorption and emission wavelengths when bound to
FAST1: 499  / 562 nm – FAST2: 499 / 558 nm

Molar absorption coefficient when bound to
FAST1: 48,000 – FAST2: 46,000 – frFAST: 55,000 M–1cm–1

Fluorescence quantum yield when bound to
FAST1: 49 % – FAST2: 57 % – frFAST: 50%

Affinity constant at 25°C of its complex with
FAST1: 0.08 µM – FAST2: 0.06 µM – frFAST: 0.80 µM

TFCoral

ref: 516600-250

A red, membrane-permeant, fluorogenic ligand

a.k.a. HBR-3,5DOM

Absorption and emission wavelengths when bound to
FAST1: 518  / 600 nm – FAST2: 516 / 600 nm – frFAST: 525 / 600 nm

Molar absorption coefficient when bound to
FAST1: 39,000 – FAST2: 38,000 – frFAST: 51,000 M–1cm–1

Fluorescence quantum yield when bound to
FAST1: 31 % – FAST2: 40 % – frFAST: 19%

Affinity constant at 25°C of its complex with
FAST1: 0.97 µM – FAST2: 0.41 µM – frFAST: 3.9 µM

TFPoppy

ref: 555670-250

A far-red, membrane-permeant, fluorogenic ligand

a.k.a. HPAR-3OM

Absorption and emission wavelengths when bound to
frFAST: 555  / 670 nm

Molar absorption coefficient when bound to
frFAST: 45,000 M–1cm–1

Fluorescence quantum yield when bound to
frFAST: 21 %

Affinity constant at 25°C of its complex with
frFAST: 1.0 µM

TFAmber-NP

ref: 505559-250

A yellowish, non-membrane permeant, fluorogenic ligand

a.k.a. HBRAA-3E

Absorption and emission wavelengths when bound to
FAST1: 505  / 559 nm

Molar absorption coefficient when bound to
FAST1: 61,000 M–1cm–1

Fluorescence quantum yield when bound to
FAST1: 8 %

Affinity constant at 25°C of its complex with
FAST1: 1.3 µM

TFFox-NP

ref: 524578-250

A red, non-membrane permeant, fluorogenic ligand

a.k.a. HBR-3,5DM

Absorption and emission wavelengths when bound to
pFAST: 524  / 578 nm

Molar absorption coefficient when bound to
pFAST: 58,000 M–1cm–1

Fluorescence quantum yield when bound to
pFAST: 22 %

Affinity constant at 25°C of its complex with
pFAST: 1.8 µM

TFDarth

ref: 5140000-250

A dark, non-fluorogenic, ligand (a fluorescence quencher)

a.k.a. HBIR-3M

Affinity constant at 25°C of its complex with
pFAST: 0.005 µM

cognate proteic tags

A further key criterion for selecting the proper Fluorogen is the tag(s) that you are implementing.  Most of our commercial Fluorogens can be used with any version of FAST (i.e., FAST1, FAST2, pFAST, tdFAST1, tdFAST2, frFAST) or splitFAST.  Besides, greenFAST is specific for TFLime and redFAST for TFCoral.  Used together, they hence allow multicolor orthogonal labeling.  frFAST was specifically designed with TFPoppy for far-red labeling.

The herebelow table provides guidance and references for helping your choice.

 

FAST1, a.k.a. Y-FAST – more about FAST1 sequence

FAST2, a.k.a. iFAST – more about FAST2 sequence

tdFAST1 – more about tdFAST1 sequence

tdFAST2 – more about tdFAST2 sequence

frFAST – more about frFAST sequence

greenFAST – more about greenFAST sequence

redFAST – more about  redFAST sequence

FAST tags X Fluorogens

 
FAST1
FAST2
pFAST
tdFAST1
tdFAST2
splitFAST
redFAST
greenFAST
frFAST
split-frFAST
 
a.k.a. Y-FAST
a.k.a. iFAST
optimized FAST
FAST1 tandem
FAST2 tandem
FAST1 split
for red labelling
for green labelling
for far-red labelling
split for far-red labelling
TFLime
a.k.a. HMBR
480-541 nm

+++

+++

+++

+++

+++

+++

0

+++

+

+

PNAS
2016
TFAmber
a.k.a. HBR-3,5DM
499-558 nm

+++

+++

+++

+++

+++

+++

0

0

+

+

Chem. Sci.
2017
TFAmber-NP
a.k.a. HBRAA-3E
505-559 nm
non permeant

+++

+++

+++

+++

+++

+++

0

0

+

+

Bioconjug. Chem.
2018
TFFox-NP
a.k.a. HBRAA-3,5DM
524-578 nm
non permeant

n.d.

n.d.

+++

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

Nat. Commun.
2021
TFCoral
a.k.a. HBR-3,5DOM
516-600 nm

+++

+++

+++

+++

+++

+++

+++

0

+

+

Chem. Sci.
2017
TFPoppy
a.k.a. HPAR-3OM
555-670 nm

0

0

0

0

0

0

0

0

+++

+++

Angew. Chem. Int. Ed.
2020
TFDarth
a.k.a. HBIR-3M
non fluorescent

n.d.

n.d.

+++

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

Nat. Commun.
2021
PNAS 2016Biochemistry 2018 Nat. Commun. 2021 Biochemistry 2018 Biochemistry 2018 Nat. Commun. 2021 Nat. Chem. Biol. 2020 Nat. Chem. Biol. 2020 Angew. Chem. Int. Ed. 2020
+++ perfect fit+ average0 NOKn.d. no data
This table allows for selecting the best couple FAST tag X Fluorogen depending on your application

Open as PDF

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