frFAST plasmids are now available from Arnaud Gautier Lab Plasmids at Addgene repository.


tfPoppy and frFAST for far-red labeling with The Twinkle Factory

Prof. Arnaud Gautier has recently disclosed frFAST and split-frFAST, variants of FAST and splitFAST, in Angew. Chem. Int. Ed. 2020.  frFAST, combined with the fluorogen HPAR-3OM (tfPoppy of The Twinkle Factory), allows for far-red protein tagging.  frFAST is hence related to the RFP (red fluorescence proteins) family.  frFAST and tfPoppy build up on FAST legacy: instantaneous, reversible, oxygen-free.  They are hence unique for spectral multiplexing and deep tissue imaging.  Moreover, Gautier has developed a complementation version, split-frFAST, hence allowing for tracking transient protein-protein interactions.

Thereafter, to help the life science research community expedite their research, Gautier has made available on Addgene seven frFAST and split-frFAST plasmids.  They add to the existing plasmids for FAST, splitFAST, redFAST and greenFAST, making together as a result a collection of 40+ plasmids.

About The Twinkle Factory

The Twinkle Factory develops the fluorogens for the tag FAST and its variants: splitFAST, frFAST, split-frFAST, greenFAST and redFAST.  These small fluorogenic molecules are indeed required as the protein tags don’t fluoresce, neither fluorogens, until bound together.  As a result, the pair tag:fluorogen then reveals the protein of interest.  Incorporated in Paris, France in 2017, it started selling fluorogens early 2019.  Thereafter, sales have been rapidly spreading worldwide.  The growing catalog of The Twinkle Factory includes tfPoppy (HPAR-3OM), specific for frFAST and split-frFAST.

About Addgene

Addgene is a nonprofit plasmid repository.  Its mission is to accelerate research and discovery by improving access to DNA-based research reagents and associated data to scientists worldwide.  Addgene hence collects material from researchers all around the world.  As a result, Addgene’s repository today contains over 70,000 plasmids.  They include special collections on CRISPR, fluorescent proteins, chemogenetics, optogenetics, and viral plasmids.