Jun 13, 2022 | Anaerobe labeling, FAST and splitFAST
FAST and splitFAST in Archaea! J. Bacteriol. 2022 just reports a team of the University of Minnesota implementing those in Methanococcus maripaludis. Furthering bacteria and eukaryots, this step opens a brand new domain for The Twinkle Factory. Terry Papoutsakis of...
Feb 21, 2022 | FAST and splitFAST, Fluorogens, The Twinkle Factory
The membrane non-permeant fluorogen TFFox-NP adds to the ever-expanding toolkit of The Twinkle Factory. Indeed, it is coming after the dark chromophore TFDarth just released, and the far-red fluorogen TFPoppy in 2020. TFFox-NP belongs to the group of membrane...
Feb 14, 2022 | FAST and splitFAST, Fluorogens, The Twinkle Factory
The dark chromophore tfDarth adds an exciting novel feature to the ever-expanding toolkit of The Twinkle Factory. tfDarth indeed forms a very tight, non-fluorescent assembly with pFAST. pFAST is the recently disclosed promiscuous variant of the now well-known FAST...
Feb 7, 2022 | Anaerobe labeling, FAST and splitFAST
Strain engineering with FAST is getting a hot topic in the anaerobe community these days. A team led by Dr. Frank Bengelsdorf of Ulm University has just added Acetobacterium woodii to the continuously expanding list of FAST-tagged anaerobes. They indeed just...
Jan 31, 2022 | Anaerobe labeling, FAST and splitFAST
greenFAST and redFAST open the door for multicolor approaches to investigate cell dynamics, gene expression, or protein localization in anaerobes. This is the conclusion of Maximilian Flaiz et al. in this ACS Synth. Biol. 2022 paper. Indeed, researchers of Prof. Dr....
Jan 31, 2022 | FAST and splitFAST, Fluorogens
Prof. Arnaud Gautier continues leading the development of amazing features of FAST and splitFAST. He, and collaborators, indeed just published in Nat. Commun. 2021 a novel “promiscuous” variant, pFAST. Painting with pFAST is the new motto! As a result...
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