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splitFAST reports complex IV assembly in single yeast cells

Oct 26, 2026 | FAST and splitFAST

A split reporter can tell whether a protein complex has been built, cell by cell. A preprint from Harvard uses splitFAST with TFLime to read out the assembly of cytochrome c oxidase (complex IV) in the mitochondria of living yeast, and the readout helps explain why...

splitFAST times ER-mitochondria contacts in liver disease

Oct 21, 2026 | FAST and splitFAST

A reversible split reporter can tell how long an organelle contact lasts, not just whether it formed. A new study in The Journal of Clinical Investigation used splitFAST with TFLime to count contacts between the endoplasmic reticulum (ER) and mitochondria in live...

Photoconvertible FAST: a label whose colour you switch with light

Oct 6, 2026 | FAST and splitFAST

Light can now change the colour of a FAST label, and change it back. A new study shows that FAST tags researchers already use, combined with the right fluorogens, become reversible photoswitches across the whole visible range, and even reversibly photoconvertible...

Recent Posts

  • splitFAST reports complex IV assembly in single yeast cells
  • splitFAST times ER-mitochondria contacts in liver disease
  • Photoconvertible FAST: a label whose colour you switch with light
  • FLIM-FRET with a built-in reference: a FAST acceptor you switch on with a fluorogen
  • Turning fluorescence on and off: our Labcompare article on reversible tags

Recent Comments

  • match715, a near-infrared dimerizer for CATCHFIRE, now available on CATCHFIRE, a brandnew self-reporting protein dimerizer
  • frFAST for far-red labeling disclosed by Prof. Arnaud Gautier on The Twinkle Factory launches tfPoppy for far-red labeling
  • FAST in Thermoanaerobacter kivui - continued on Painting with pFAST, a FAST variant, for advanced imaging
  • Secretory system of Clostridium perfringens with FAST on Visualizing bacterial protein export with FAST
  • FAST in Saccharomyces cerevisiae, a routine technique! on Metabolic engineering in S. cerevisiae with FAST – Cont’d

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The Twinkle Factory

French biotech supplying fluorogens for the FAST & splitFAST protein tags and fluorogenic molecular glues for CATCHFIRE, chemogenetic reagents for reversible fluorescent labelling and live-cell & in vivo imaging.

© 2026 Twinkle Bioscience S.A.S.

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